Appendix 11 Set-up and take-down of seawater supply in algae container

Set-up of seawater supply in algae container:
Note: Refer to Technical Drawings – Pg 1 and Pg 9.
1. Remove filters and hose from chlorinated bin.

2. Rinse abundantly with fresh water all filters (coarse, 10 μm and 1 μm) and flexible water hose.
3. Set-up filters in respective cartridges fixed to outside of container, and connect flexible hose to UV sterilizer placing end in sink of algae container.
4. Open drain valve Z by Y-junction and allow chlorinated water to drain. Open union by inflow valve Q to coarse filter and rinse abundantly with fresh water.
5. Close drain valve Z and union.
6. Open inflowing valve Q to coarse filter all the way and close outflowing valve O of coarse filter.
7. Slightly open air vent of coarse filter.
8. Open main valve Y to algal container (from Y-junction) three quarters of the way. 9. Allow coarse filter to fill with water.
10. When water starts to come out of air vent (it takes a few minutes only), open outflow valve O halfway to allow water to pass through 10 and 1 μm filters.

11. Check that water is passing through UV sterilizer and flowing out of flexible hose into sink. Let run for 15 minutes to eliminate all residue of chlorine.
12. Plug UV sterilizer in when ready to use. Control seawater flow with main valve Y and outflow valve O.
Take down of seawater supply to algae container:
Note: Refer to Technical Drawings – pages 1 and 9.
1. Unplug UV sterilizer.
2. Close main valve K at Y-junction.
3. Close inflow valve I to coarse filter and open drain plug of coarse filter. Allow coarse filter to empty.
4. Remove filters from coarse 10 μm and 1 μm cartridges. Rinse salt off with fresh water and place in chlorinated bin until next use. Do the same with flexible hose.
5. Rinse coarse filter with fresh water allowing to empty via drain plug. Rinse lid as well and place back on coarse filter.
6. Undo union prior to inflow valve I and open drain valve H of pipe adjacent to Y-junction.
7. Blast fresh water through pipe for a few minutes. Close drain valve H. Fill pipe with fresh water and a capful of chlorox. Close union and let sit until next use.
8. Undo union after 10 and 1 μm cartridges and rinse with fresh water hose for a few minutes to rinse line through UV sterilizer with fresh water. Make sure to place a tray under UV sterilizer to collect fresh water and prevent spilling on algal container floor.
9. Allow pipe to drain. Rinse 10 and 1 μm cartridges with fresh water and replace on-line.